Overwintering management on upland pasture causes shifts in an abundance of denitrifying microbial communities, their activity and N.sub.2./sub.O-reducing ability

0328468 - BC 2010 RIV GB eng J - Journal Article
Chroňáková, Alica - Radl, V. - Čuhel, Jiří - Šimek, Miloslav - Elhottová, Dana - Engel, M. - Schloter, M.
Overwintering management on upland pasture causes shifts in an abundance of denitrifying microbial communities, their activity and N₂O-reducing ability.
Soil Biology and Biochemistry. Roč. 41, č. 6 (2009), s. 1132-1138. ISSN 0038-0717
Institutional research plan: CEZ:AV0Z60660521
Keywords : nitrous oxide * qPCR * DEA
Subject RIV: EH - Ecology, Behaviour
Impact factor: 2.978, year: 2009

Pasture soils used for cattle overwintering may represent significant sources of N₂O emissions from soils. Therefore, the long-term effect of cattle overwintering on the abundance and activity of a denitrifying community was explored. The study was performed at a cattle overwintering area in South Bohemia (Czech Republic), where three sites differing in the degree of animal impact were selected: severely impacted (SI) and moderately impacted (MI), as well as a control site with no impact (NI). N₂O flux measurement and soil sampling were performed in spring and fall of 2005. The activity was measured in terms of potential denitrification activity. Bacterial nirK, nirS and nosZ genes were used as functional markers of the denitrifying communities; abundance was analyzed using a real-time PCR assay.
Permanent Link: http://hdl.handle.net/11104/0174776