Cbf11 and Cbf12, the fission yeast CSL proteins, play opposing roles in cell adhesion and coordination of cell and nuclear division

0328355 - MBÚ 2010 RIV US eng J - Journal Article
Převorovský, M. - Groušl, Tomáš - Staňurová, J. - Ryneš, J. - Nellen, W. - Půta, F. - Folk, P.
Cbf11 and Cbf12, the fission yeast CSL proteins, play opposing roles in cell adhesion and coordination of cell and nuclear division.
Experimental Cell Research. Roč. 315, č. 8 (2009), s. 1533-1547. ISSN 0014-4827. E-ISSN 1090-2422
R&D Projects: GA ČR(CZ) GD204/03/H066
Grant - others:UK(CZ) 157/2005/B-BIO/PrF
Institutional research plan: CEZ:AV0Z50200510
Keywords : csl family * fission yeast * adhesion
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.589, year: 2009

The CSL (CBF1/RBP-J/Suppressor of Hairless/LAG-1) family is comprised of transcription factors essential for metazoan development, mostly due to their involvement in the Notch receptor signaling pathway. Recently, we identified two novel classes of CSL genes in the genomes of several fungal species, organisms lacking the Notch pathway. In this study, we characterized experimentally cbf11plus and cbf12plus, the two CSL genes of Schizosaccharomyces pombe, in order to elucidate the CSL function in fungi. We provide evidence supporting their identity as genuine CSL genes. Both cbf11plus and cbf12plus are non-essential, they have distinct expression profiles and code for nuclear proteins with transcription activation potential. Significantly, we demonstrated that Cbf11 recognizes specifically the canonical CSL response element GTGA/GGAA in vitro
Permanent Link: http://hdl.handle.net/11104/0174687