Enzymatic preparation of acylated derivatives of silybin in organic and ionic liquid media and evaluation of their antitumor proliferative aktivity

0328344 - MBÚ 2010 RIV GB eng J - Journal Article
Theodosiou, E. - Katsoura, M. H. - Loutrari, H. - Purchartová, Kateřina - Křen, Vladimír - Kolisis, F. N. - Stamatis, H.
Enzymatic preparation of acylated derivatives of silybin in organic and ionic liquid media and evaluation of their antitumor proliferative aktivity.
[Enzymová příprava acylovaných derivátů silybinu v organických a iontových tekutých médiích a hodnocení jejich antitumorické proliferativní aktivity.]
Biocatalysis and Biotransformation. Roč. 27, č. 3 (2009), s. 161-169. ISSN 1024-2422. E-ISSN 1029-2446
R&D Projects: GA MŠMT ME 922
Grant - others:GA MŠk(CZ) Czech - Greece Research and Technology Program: Kontakt 7-2006-16
Institutional research plan: CEZ:AV0Z50200510
Keywords : silybin * lipase * ionic liquids
Subject RIV: CE - Biochemistry
Impact factor: 1.017, year: 2009

Biocatalytic preparation of acylated derivatives of silybin using several acyl donors (free fatty acids or their esters), catalyzed by immobilized Candida antarctica lipase B, was performed in various organic solvents as well as in imidazolium-based ionic liquids containing either BF-4 or PF-6 anions. Total conversion of silybin was achieved in acetone using short-chain acyl donors (vinyl butanoate) at a 10:1 molar ratio of acyl donor to silybin. The enzymatic process in ionic liquids depended strongly on the alkyl chain length of the cation as well the anion used. Higher conversion yields (up to 75.8 percent) and reaction rates (up to 0.31 mmol h-1 g-1 biocatalyst) were obtained in the BF-4 as compared with PF-6 - containing ionic liquids

Práce se zabývá enzymovou přípravou acylovaných derivátů silybinu v organických a iontových letkutých médiích a hodnocením jejich antitumorické proliferativní aktivity. Byla použita katalýza immobilizovanou lipasou B z Candida antarctica v imidazoliových iontových tekutinách s BF-4 nebo PF-6 anionty
Permanent Link: http://hdl.handle.net/11104/0174677