Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases

0328051 - MBÚ 2010 RIV NL eng J - Journal Article
Syslová, K. - Kačer, P. - Kuzma, Marek - Najmanová, V. - Fenclová, Z. - Vlčková, Š. - Lebedová, J. - Pelclová, D.
Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases.
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 877, č. 24 (2009), s. 2477-2486. ISSN 1570-0232. E-ISSN 1873-376X
R&D Projects: GA MZd NR9338
Institutional research plan: CEZ:AV0Z50200510
Keywords : malondialdehyde * 4-Hydroxynonenal * exhaled breath condensate
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.777, year: 2009

Sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F2a, malondialdehyde and 4-hydroxynonenal. The method consisted of a pre-treatment part a solid-phase extraction, for rapid and effective isolation of biomarkers from body fluids (exhaled breath condensate, plasma and urine) and the detection method LC–ESI-MS/MS, where the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method was characterized by the following parameters: the imprecision was below 14.3 percent, the mean inaccuracy was determined to be lower than 13.1 percent
Permanent Link: http://hdl.handle.net/11104/0174459