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Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods
- 1.0326498 - ÚOCHB 2010 RIV US eng J - Journal Article
Klusák, Vojtěch - Bařinka, C. - Plechanovová, Anna - Mlčochová, Petra - Konvalinka, Jan - Rulíšek, Lubomír - Lubkowski, J.
Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods.
Biochemistry. Roč. 48, č. 19 (2009), s. 4126-4138. ISSN 0006-2960
R&D Projects: GA MŠMT 1M0508; GA MŠMT LC512
Institutional research plan: CEZ:AV0Z40550506
Keywords : reaction mechanism * QM/MM calculations * glutamate carboxypeptidase II * X-ray crystallography * mutant activity
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 3.226, year: 2009
The peptide hydrolysis by GCPII was investigated in detail by carrying out the kinetic analysis of GCPII(E424A) using N-Ac-Asp-Glu as substrate. The crystal structure of GCPII(E424A) in complex with N-Ac-Asp-Glu was determined at 1.70 A resolution. The experimental data were then complemented by the QM/MM calculations which provided detailed information concerning the GCPII reaction mechanism.
Permanent Link: http://hdl.handle.net/11104/0173587
Number of the records: 1