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Detection of extracellular phosphatase activity at the single-cell level by Enzyme-Labeled Fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling
- 1.0326242 - BC 2010 RIV US eng J - Journal Article
Duhamel, S. - Gregori, G. - Van Wambeke, F. - Nedoma, Jiří
Detection of extracellular phosphatase activity at the single-cell level by Enzyme-Labeled Fluorescence and flow cytometry: The importance of time kinetics in ELFA labeling.
[Detekce extracelulární fosfatázové aktivity na úrovni jednotlivých buněk metodou Enzyme-Labeled Fluorescence a průtokovou cytometrií.]
Cytometry. Part A. 75A, č. 2 (2009), s. 163-168. ISSN 1552-4922. E-ISSN 1552-4930
Grant - others:MŠMT(CZ) PAI Barrande 2005-06-009-01
Institutional research plan: CEZ:AV0Z60170517
Keywords : alkaline phosphatase * ELF phosphate * heterotrophic bacteria
Subject RIV: DA - Hydrology ; Limnology
Impact factor: 3.032, year: 2009
ELF97 phosphate (ELF-P) is a useful compound for assessing the phosphorus-related status of planktonic aquatic populations. We have used a recently developed protocol that enables the detection by flow cytometry of ELF alcohol (ELFA), the product of ELF-P hydrolysis. The percentage of ELFA-labeled cells, the relative median ELFA fluorescence per cell, and the absolute ELFA fluorescence were determined in both freshwater (lake) and marine samples.
ELF97 fosfát (ELF-P) je použitelný pro hodnocení stavu populací vodních organismů z hlediska metabolizmu fosforu. Použili jsme recentně vyvinutý postup který umožňuje detekci ELF alkoholu (ELFA), produktu hydrolýzy ELF-P. Ve sladkovodních a mořských vzorcích jsme stanovili procento buněk značených ELFA, relativní median fluorescence ELFA a absolutní fluorescenci ELFA.
Permanent Link: http://hdl.handle.net/11104/0173402
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