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Faithful inheritance of cytosine methylation patterns in repeated sequences of the allotetraploid tobacco correlates with the expression of DNA methyltransferase gene families from both parental genomes

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    0323254 - BFÚ 2009 RIV US eng J - Journal Article
    Fulneček, Jaroslav - Matyášek, Roman - Kovařík, Aleš
    Faithful inheritance of cytosine methylation patterns in repeated sequences of the allotetraploid tobacco correlates with the expression of DNA methyltransferase gene families from both parental genomes.
    [Spolehlivá dědičnost vzoru methylace cytosinů v repetitivních sekvencích allotetraploidního tabáku koreluje s expresí genových rodin DNA methyltransferas z obou rodičovských genomů.]
    Molecular Genetics and Genomics. Roč. 281, č. 4 (2009), s. 407-420. ISSN 1617-4615. E-ISSN 1617-4623
    R&D Projects: GA ČR(CZ) GA204/06/1432; GA ČR(CZ) GA521/07/0116
    Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
    Keywords : cytosine methylation * DNA (cytosine-5) methyltransferase * allopolyploidy
    Subject RIV: BO - Biophysics
    Impact factor: 2.579, year: 2009

    Complementary DNA sequences of three main plant DNA (cytosine-5) methylatransferase genes (MET1, CMT3, DRM) were cloned and sequenced from Nicotiana sylvestris and Nicotiana tomentosiformis, maternal and paternal progenitors of tobacco (Nicotiana tabacum) respectively. All methyltransferase parental genes had been inherited in tobacco where they were additively expressed in all examined tissues. Methylation analysis of several repetitive sequences showed similar levels of 5-methylcytosine in tobacco and its progenitor species.

    Komplementární DNA sekvence tří hlavních rostlinných genů DNA (cytosin-5) methylatransferas (MET1, CMT3, DRM) byly klonovány a sekvenovány z Nicotiana sylvestris a Nicotiana tomentosiformis,mateřských a otcovských progenitorů tabáku (Nicotiana tabacum). Všechny rodičovské geny byly v tabáku zděděny, a zde byly aditivně exprimovány ve všech zkoumaných pletivech. Methylační analysa několika repetitivních sekvencí ukázala podobné hladiny 5-methylcytosinů v tabáku a jeho progenitorových druzích.
    Permanent Link: http://hdl.handle.net/11104/0171269

     
     
Number of the records: 1  

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