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Evaluation of the possible proteomic application of trypsin from Streptomyces griseus
- 1.0312520 - ÚIACH 2009 RIV US eng J - Journal Article
Štosová, T. - Šebela, M. - Řehulka, Pavel - Šedo, O. - Havliš, J. - Zdráhal, Z.
Evaluation of the possible proteomic application of trypsin from Streptomyces griseus.
[Vyhodnocení možných proteomických aplikací trypsinu ze Streptomyces griseus.]
Analytical Biochemistry. Roč. 376, č. 1 (2008), s. 94-102. ISSN 0003-2697. E-ISSN 1096-0309
Institutional research plan: CEZ:AV0Z40310501
Keywords : MALDI-TOF MS * Streptomyces griseus * trypsin
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.088, year: 2008
In this work, trypsin from Streptomyces griseus (SGT) was purified to homogeneity from pronase. The enzyme was evaluated in in-gel digestion of protein standards followed by matrix-assisted laser desorption/ionization time-of-flight (MALDI–TOF) mass spectrometry (MS) analyses of the digests.
V této práci byl z pronázy Streptomyces griseus čištěn trypsin. Enzym byl stanovován in-gel digescí standardních proteinů a analýzou digestů pomocí matrix-assisted laser desorption/ionization time-of-flight (MALDI–TOF) hmotnostní spektrometrie (MS).
Permanent Link: http://hdl.handle.net/11104/0163565
Number of the records: 1