0308463 - BTÚ 2008 RIV GB eng J - Journal Article
Stahlberg, A. - Elbing, K. - Andrade-Garda, J.M. - Sjögreen, B. - Forootan, A. - Kubista, Mikael
Multiway real-time PCR gene expression profiling in yeast. Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli.
[Vícecestné expresní profilování PCR v reálném čase v kvasinkách Saccharomyces cerevisiae objevilo změněnou transktipční odpověď ADH-genů na stimulaci glokózou.]
BMC Genomics. Roč. 9, č. 170 (2008), s. 1-41. ISSN 1471-2164. E-ISSN 1471-2164
Institutional research plan: CEZ:AV0Z50520701
Keywords : Expression Profiling * Real-time PCR * Yeast
Subject RIV: EI - Biotechnology ; Bionics
Impact factor: 3.926, year: 2008 ; AIS: 1.624, rok: 2008

The large sensitivity, high reproducibility and essentially unlimited dynamic range of real-time PCR to measure gene expression in complex samples provides the opportunity for powerful multivariate and multiway studies of biological phenomena. In multiway studies samples are characterized by their expression profiles to monitor changes over time, effect of treatment, drug dosage etc. Here we perform a multiway study of the temporal response of four yeast Saccharomyces cerevisiae strains with different glucose uptake rates upon altered metabolic conditions

We measured the expression of 18 genes as function of time after addition of glucose to four strains of yeast grown in ethanol. The data are analyzed by matrix-augmented principal component analysis, which is a generalization of principal component analysis for 3-way data, and the results are confirmed by hierarchical clustering and clustering by Kohonen self-organizing map. Our approach identifies gene groups that respond similarly to the change of nutrient, and genes that behave differently in mutant strains. Of particular interest is our finding that ADH4 and ADH6 show a behavior typical of glucose-induced genes, while ADH3 and ADH5 are repressed after glucose addition
Permanent Link: http://hdl.handle.net/11104/0160942