Detection of apoptosis in paraffin embedded tissues: the influence of tissue type and fixation

0213494 - UZFG-Y 20023110 RIV CZ eng J - Journal Article
Matalová, Eva - Dubská, L. - Míšek, Ivan
Detection of apoptosis in paraffin embedded tissues: the influence of tissue type and fixation.
Acta veterinaria Brno. Roč. 71, č. 4 (2002), s. 529-533. ISSN 0001-7213. E-ISSN 1801-7576
R&D Projects: GA ČR GP204/02/P112; GA AV ČR KSK6005114
Keywords : apoptosis * TUNEL test * paraffin embedded tissues
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.370, year: 2002

The influence of different ways of fixation on TUNEL POD results was investigated. Three commonly employed fixative procedures using formol, methacarn and Bouin liquid were applied. Two bovine tissue types with different cell density (thymus and ovary) were used to test this effect. Different pretreatments were tested in case of false negative findings. Results were evaluated under light microscopy and photodocumented.
Permanent Link: http://hdl.handle.net/11104/0109030