Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes

0213221 - UZFG-Y 20003009 RIV US eng J - Journal Article
Motlík, Jan - Pavlok, Antonín - Lapathitis, Georgios - Kubelka, Michal
Impact of Two-step In Vitro Culture Systems on Developmental Potency of Oocytes.
Reproduction in Domestical Animals. Roč. 35, - (2000), s. 267-271. ISSN 0936-6768. E-ISSN 1439-0531
R&D Projects: GA ČR GV524/96/K162; GA AV ČR KSK2038602
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.521, year: 2000

Proper conditions for the successful induction of a functional two step in vitro culture system for agricultural or clinical applications are hereby described in form of a two-step protocol. The first inhibitory culture of oocytes in media supplemented with specific cdk inhibitors (roscovitine, BL I) appears essential to promote appropriate oocyte-cumulus cell interactions and ensure production of ova competent to undergo normal embryogenesis. This inhibitory period should last for several days, depending on the origin of the follicles, and the degree of accumulation and storage of mRNAs and proteins essential for the acquisition of developmental competence in the oocytes. The full reversibility of the inhibitory step secures that during the second maturation step, oocytes reach metaphase IT with correctly arranged microtubuli in the meiotic spindle and well-expanded cumuli.
Permanent Link: http://hdl.handle.net/11104/0108759