Study of PCB degradation in real contaminated soil

0191686 - UMG-J 20033201 RIV DE eng J - Journal Article
Ryšlavá, E. - Krejčík, Zdeněk - Macek, Tomáš - Nováková, H. - Demnerová, K. - Macková, M.
Study of PCB degradation in real contaminated soil.
Fresenius Environmental Bulletin. Roč. 2003, č. 12 (2003), s. 296-301. ISSN 1018-4619. E-ISSN 1610-2304
R&D Projects: GA MŠMT LN00A079; GA ČR GA526/01/1292
Grant - others:GA EU(XE) QLK3-CT-2001-00101
Institutional research plan: CEZ:AV0Z4055905; CEZ:AV0Z5052915
Keywords : Phytoremediation * rhizoremediation * PCB degradation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.325, year: 2003

In this study three different plant species were cultivated in contaminated soil with PCBs. After 6 months the decrease of PCB content in soil, PCBs accumulation into plants and the changes in participation of rhizospheric microorganisms in vegetated and non-vegetated soil were analysed. From polluted soil also bacteria which potentially participate in biodegradation of PCBs were isolated. They were tested for the presence of known genes of biphenyl operon. These tests were based on PCR amplification of bphA1 and bphC genes. The degradative capabilities were confirmed in presence of biphenyl as cosubstrate under the laboratory conditions. Strains, showing the best growth and degradative properties were taxonomically identified by determination of nucleotide sequence of 16S ribosomal DNA.
Permanent Link: http://hdl.handle.net/11104/0087423