D-fructose-binding proteins in bull seminal plasma: Isolation and characterization

0191223 - UMG-J 20010294 RIV CZ eng J - Journal Article
Liberda, J. - Kraus, Marek - Ryšlavá, H. - Vlasáková, M. - Jonáková, Věra - Tichá, M.
D-fructose-binding proteins in bull seminal plasma: Isolation and characterization.
Folia Biologica. Roč. 47, č. 4 (2001), s. 113-119. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA ČR GA303/99/0357; GA ČR GV524/96/K162
Institutional research plan: CEZ:AV0Z5052915
Keywords : bull seminal plasma * non-heparin-binding and heparin-binding proteins * D-fructose-binding proteins
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.519, year: 2001

The heparin-binding activity of bull seminal plasma proteinswas inhibited by D-fructose, D-glucose, inulin and glycogen. The heparin-binding activity of epididymal sperm was inhibited by D-fructose. The results of the binding studies were confirmed by affinity chromatography on immobilized heparin followed by elution with monosaccharides. Proteins eluted with D-fructose were analyzed by N-terminal amino acid sequence. RNAase dimer, PDC-109 and metalloproteinase inhibitor were identified.
Permanent Link: http://hdl.handle.net/11104/0086980