Mannosides as crucial part of bioactive supports for cultivation of human epidermal keratinocytes without feeder cells

0189059 - UMCH-V 20033011 RIV NL eng J - Journal Article
Labský, Jiří - Dvořánková, B. - Smetana, Karel - Holíková, Z. - Brož, L. - Gabius, H.J.
Mannosides as crucial part of bioactive supports for cultivation of human epidermal keratinocytes without feeder cells.
Biomaterials. Roč. 24, č. 5 (2003), s. 863-872. ISSN 0142-9612. E-ISSN 1878-5905
R&D Projects: GA ČR GA203/00/1310; GA MŠMT LN00A065; GA MZd ND6340
Institutional research plan: CEZ:AV0Z4050913
Keywords : cell therapy * keratinocyte * mannose
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 2.903, year: 2003

Large-scale production of keratinocytes by cell culture is of interest for medical applications. Long-term cultivation of epidermal cells is presently possible with feeder cells, i.e. 3T3 fibroblasts with arrested mitosis, or with specially formulated culture medium. To define refinements for in vitro conditions, the analysis of the natural environment with growth-maintaining/stimulating factors can provide important clues. Cells with proliferative activity are located in the basal layer of the epidermis in close contact with a basement membrane.
Permanent Link: http://hdl.handle.net/11104/0002612