Comparative Measurements of Total Body Water in Healthy Volunteers by On-line Breath Deuterium Measurement and other Near-subject Methods

0181625 - UFCH-W 20030039 RIV US eng J - Journal Article
Smith, D. - Engel, B. - Diskin, A. M. - Španěl, Patrik - Davies, S. J.
Comparative Measurements of Total Body Water in Healthy Volunteers by On-line Breath Deuterium Measurement and other Near-subject Methods.
American Journal of Clinical Nutrition. Roč. 76, - (2002), s. 1295-1301. ISSN 0002-9165. E-ISSN 1938-3207
R&D Projects: GA ČR GA203/00/0632
Institutional research plan: CEZ:AV0Z4040901
Keywords : measurements * bioimpedance analysis
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 5.601, year: 2002

Background: Accurate and rapid determination of total body water (TBW), a fundamental component of body composition, is a desirable goal in the study of physiology and the management of many medical conditions. We have developed a new breath test, using Flowing-afterglow mass spectrometry (FA-MS) and deuterium dilution, which enables the immediate determination of TBW from single exhalations. Objective: To demonstrate the efficacy of the new FA-MS method in determining TBW in healthy subjects, and compare the results obtained with those from multifrequency bioimpedance analysis (BIA) and published regression equations. Design: Following baseline measurement of breath deuterium abundance (FA-MS), 24 healthy subjects took 0.3g/kg body weight D2O. A second breath sample was taken after two hours to measure the increase in deuterium and thus calculate TBW. BIA was carried out using a Xitron Hydra (4200). Methods were compared using Pearson correlation and Bland-Altman analyses.
Permanent Link: http://hdl.handle.net/11104/0078161