Comparison of cytokinin-binding proteins from wheat and oat grains

0172370 - UEB-Q 20033078 RIV DK eng J - Journal Article
Kamínek, Miroslav - Trčková, M. - Fox, J. E. - Gaudinová, Alena
Comparison of cytokinin-binding proteins from wheat and oat grains.
Physiologia Plantarum. Roč. 117, č. 4 (2003), s. 453-458. ISSN 0031-9317. E-ISSN 1399-3054
R&D Projects: GA ČR GV206/96/K188; GA ČR GA522/02/0530
Institutional research plan: CEZ:AV0Z5038910
Keywords : Cytokinin-binding proteins * Avena sativa * SDS-polyacrylamide gel
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.767, year: 2003

Cytokinin-binding proteins (CBPs) isolated from mature grains of oat (Avena sativa L.) and wheat (Triticum aestivum L.) by acid precipitation, ion-exchange and affinity chromatography had similar characteristics, although they differed somewhat in apparent molecular weight of the native protein as determined by gel filtration (109 and 133 kDa, respectively) and subunit size as estimated by SDS-polyacrylamide gel electrophoresis (47 and 55 kDa, respectively). Highly purified oat CBP showed very weak but distinct immunochemical cross-reactivity with anti-wheat CBP IgG, indicating different immunogenic properties of the two CBPs. Nevertheless, both CBPs exhibited very similar binding of different cytokinins and were characterized by high affinity for N6-benzyladenine (BA)-type and by low affinity for zeatin-type cytokinins to both wheat and oat CBPs and by somewhat higher binding activities of oat CBP compared to wheat CBP (Kds for BA: 4.6 107 M and 6.8 107 M, respectively). The potential role of CBPs in regulating free BA-type cytokinin levels during cereal grain development and germination is discussed.
Permanent Link: http://hdl.handle.net/11104/0069409