Cyclin-dependent kinase inhibitor, roscovitine, in combination with exogenous cytokinin, N6-benzyladenine, causes increase of cis-cytokinins in immobilized tobacco cells

0172315 - UEB-Q 20033040 RIV NL eng J - Journal Article
Blagoeva, Elitsa - Malbeck, Jiří - Gaudinová, Alena - Vaněk, Tomáš - Vaňková, Radomíra
Cyclin-dependent kinase inhibitor, roscovitine, in combination with exogenous cytokinin, N6-benzyladenine, causes increase of cis-cytokinins in immobilized tobacco cells.
Biotechnology Letters. Roč. 25, č. 6 (2003), s. 469-472. ISSN 0141-5492. E-ISSN 1573-6776
R&D Projects: GA MŠMT OC 840.20; GA MŠMT LN00A081
Institutional research plan: CEZ:AV0Z4055905; CEZ:AV0Z5038910
Keywords : alginate * cytokinins * plant cell immobilization
Subject RIV: ED - Physiology
Impact factor: 0.778, year: 2003

Dynamics of the response of tobacco cells (line BY-2) to exogenous cytokinin, N6-benzyladenine, and cyclin-dependent kinase inhibitor, roscovitine, was followed using alginate-immobilized cells packed into a column. N6-Benzyladenine (1.25 μM) increased the synthesis of the physiologically-active endogenous cytokinin, isopentenyladenosine, in the effluent up to 0.1 nM. Simultaneously, conversion of the excess of endogenous cytokinins to biologically inactive derivatives of cis-zeatin occurred, up to 0.8 nM. Roscovitine (50 μM) further increased cis-cytokinins, up to 2.2 nM.
Permanent Link: http://hdl.handle.net/11104/0069354