0172279 - UEB-Q 20023064 RIV US eng J - Journal Article
Vermeulen, K. - Strnad, Miroslav - Havlíček, Libor - Onckelen, H. - Lenjou, M. - Nijs, G. - Bockstaele, D. R. - Berneman, Z. N.
Plant cytokinin analogues with inhibitory activity on cyclin-dependent kinases exert their antiproliferative effect through induction of apoptosis initiated by the mitochondrial pathway: Determination by a multiparametric flow cytometric analysis.
Experimental Hematology. Roč. 30, č. 10 (2002), s. 1107-1114. ISSN 0301-472X. E-ISSN 1873-2399
R&D Projects: GA MŠMT OC 844.10
Institutional research plan: CEZ:AV0Z5038910
Keywords : PLASMA MEMBRANE * PURINE ANALOGS * CELLS
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.366, year: 2002

Regulation of the cell cycle by cyclin-dependent kinase (CDK) activity occurs at multiple levels and is often altered in human cancers. Therefore, CDK activity has been targeted for drug discovery, and a number of small molecules have now been identified as CDK inhibitors. Plant cytokinin analogues with CDK inhibitory activity and antiproliferative effects were studied to characterize the cellular basis of the cytotoxic effect. The main cellular mechanism of the antiproliferative effect of plant cytokinin analogues with CDK inhibitory activity is the induction of apoptosis. The multiparametric flow cytometric technique allowed to follow the kinetics of various aspects of apoptotic cell changes and demonstrated that cytokinin analogue-induced apoptosis starts through the mitochondrial pathway. This technique could also become of value for the rapid screening of pro-apoptotic properties of chemotherapeutic compounds.
Permanent Link: http://hdl.handle.net/11104/0069318