Isolation of melatonin by immunoaffinity chromatography

0172270 - UEB-Q 20023055 RIV NL eng J - Journal Article
Rolčík, Jakub - Lenobel, René - Siglerová, Věra - Strnad, Miroslav
Isolation of melatonin by immunoaffinity chromatography.
Journal of Chromatography-B. Roč. 25, č. 1 (2002), s. 9-15. ISSN 0378-4347
R&D Projects: GA MŠMT OC 844.10; GA ČR GA301/02/0475
Institutional research plan: CEZ:AV0Z5038910
Keywords : Melatonin
Subject RIV: CE - Biochemistry
Impact factor: 1.913, year: 2002

A single-step, highly specific and easy-to-use method was developed for isolation and purification of melatonin from complex biological matrices. Polyclonal antibodies highly specific against melatonin (with cross-reactivities with related compounds below 0.02%, except for 6-hydroxymelatonin) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of immunoaffinity gel. Melatonin recovery by the immunoaffinity method was ~95%, allowing single-step processing of samples prior to electrospray HPLCůMS analysis (with detection limit 10 fmol). The method was successfully used for determining melatonin in human serum and turned out to be better than the non-specific solid-phase extraction published earlier.
Permanent Link: http://hdl.handle.net/11104/0069309