Aromatic cytokinins in micropropagated potato plants

0172256 - UEB-Q 20023041 RIV FR eng J - Journal Article
Baroja, F. E. - Aguirreolea, J. - Martínková, Hana - Hanuš, Jan - Strnad, Miroslav
Aromatic cytokinins in micropropagated potato plants.
Plant Physiology and Biochemistry. Roč. 40, č. 3 (2002), s. 217-224. ISSN 0981-9428
R&D Projects: GA MŠMT OC 844.10; GA ČR GA301/02/0475
Institutional research plan: CEZ:AV0Z5038910
Keywords : Acclimatization * Aromatic cytokinins * Micropropagation
Subject RIV: CE - Biochemistry
Impact factor: 1.582, year: 2002

Endogenous cytokinins were studied in three micropropagated Solanum tuberosum L. cultivars (Kennebec, Turia and Jaerla) differing in survival after transplanting. Leaf and stem cytokinins were determined both in vitro and 10 d after being transferred to ex vitro conditions by a combination of high-performance liquid chromatography and enzyme-linked immunosorbent assay. Nine aromatic and nine isoprenoid type cytokinins were identified. Higher levels of total cytokinins mainly aromatics (92%) were detected in Kennebec, the cultivar showing better in vitro growth and 99% survival. On the contrary, a predominance of isoprenoid cytokinins (up to 57%) was observed after transplanting in Jaerla, the cultivar showing lower viability. Significant survival improvement was obtained in the Jaerla cultivar after addition to the culture medium of the aromatic cytokinin meta-topolin riboside (mTR). We also report here isolation and identification of this cytokinin by several sophisticated techniques including mTR-specific immunoaffinity chromatography, diode-array high-performance liquid chromatography (HPLC), and gas chromatographyůmass spectrometry of permethylated HPLC fractions. The occurrence of the aromatic cytokinins in potato plants is described for the first time.
Permanent Link: http://hdl.handle.net/11104/0069295