0172245 - UEB-Q 20023025 RIV DE eng J - Journal Article
Šindelář, Luděk - Šindelářová, Milada
Correlation of viral RNA biosynthesis with glucose-6-phosphate dehydrogenase activity and host resistance.
Planta. Roč. 215, - (2002), s. 862-869. ISSN 0032-0935. E-ISSN 1432-2048
R&D Projects: GA ČR GA522/99/1264
Institutional research plan: CEZ:AV0Z5038910
Keywords : Glucose 6 phosphate dehydrogenase * Nicotiana (viral infection) * Plant viruses
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.960, year: 2002

Changes in glucose-6-phosphate dehydrogenase (G6P DH; EC 1.1.1.49) activity caused by infection of tobacco (Nicotiana tabacum L.) leaves with potato virus Y (PVY), cucumber mosaic virus, potato virus X, tobacco rattle virus and turnip mosaic virus, the sub-cellular localisation of G6P DH isozymes in mesophyll protoplasts derived from healthy and PVY-infected tobacco leaves, as well as G6P DH control and the relationship of its isozymes with the degree of tobacco resistance to PVY multiplication, were studied. The activities of G6P DH were markedly increased in locally and systemically infected leaves and the time courses of the activity linearly correlated with those of virus multiplication. In leaves infected with PVY, the activity time courses of the crude and the partially purified G6P DH were coincident. This probably indicates the involvement of coarse regulation of the enzyme. PVY content linearly correlated with enhanced G6P DH activity in leaf discs derived from susceptible, tolerant and resistant cultivars of tobacco.
Permanent Link: http://hdl.handle.net/11104/0069284