0172244 - UEB-Q 20023024 RIV CZ eng J - Journal Article
Šindelář, Luděk - Šindelářová, Milada
Glucose-6-phosphate dehydrogenase/6-phosphogluconate dehydrogenase ratio and the glucose-6-phosphate, 6-phosphogluconate and fructose-6-phosphate contents in tobacco plants infected with potato virus Y.
Biologia Plantarum. Roč. 45, č. 4 (2002), s. 575-580. ISSN 0006-3134. E-ISSN 1573-8264
R&D Projects: GA ČR GA522/99/1264
Institutional research plan: CEZ:AV0Z5038910
Keywords : protoplast * chloroplast * cytosol
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.583, year: 2002

The ratio of activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (G6P DH/6PG DH), and the contents of glucose-6-phosphate (G6P), 6-phosphogluconate (6PG) and fructose-6-phosphate (F6P) were studied at various stages of potato virus Y (PVY) multiplication in Nicotiana tabacum cv. Samsun. G6P DH/6PG DH increased through the experiment from 0.42 to 0.53 in leaves of healthy tobacco, and up to 0.59 in PVY systemically infected leaves. However, these ratios in the ruptured protoplast preparations, and the chloroplast and cytosol fractions of healthy protoplasts were similar to that from infected ones. The ratio lower than 1, found in the healthy and/or PVY-infected leaf tissues and in the infected protoplasts as well, confirms the assumption that G6P DH is the control enzyme of oxidative pentosephosphate pathway not only in the healthy but also in the infected plants.
Permanent Link: http://hdl.handle.net/11104/0069283