0172236 - UEB-Q 20023013 RIV CZ eng J - Journal Article
Ajalin, I. - Kobza, F. - Doležel, Jaroslav
Ploidy identification of doubled chromosome number plants in Viola x wittrockiana Gams. M1-generation.
Horticultural Science. Roč. 29, č. 1 (2002), s. 35-40. ISSN 1416-6445
R&D Projects: GA AV ČR IAA6038204; GA AV ČR IBS5038104
Institutional research plan: CEZ:AV0Z5038910
Keywords : flow cytometry * polyploidisation * colchicine
Subject RIV: EB - Genetics ; Molecular Biology

The aim of this study was to develop a protocol for production of polyploid Ml-generation plants of Viola x wittrockiana Gams. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the Ml-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM.
Permanent Link: http://hdl.handle.net/11104/0069275