0172223 - UEB-Q 20023005 RIV NL eng J - Journal Article
Dobrev, Petre - Kamínek, Miroslav
Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction.
Journal of Chromatography A. Roč. 950, č. 1 (2002), s. 21-29. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR GA206/02/0967; GA ČR GA522/02/0530
Institutional research plan: CEZ:AV0Z5038910
Keywords : cytokinins * abscisic acid * extraction
Subject RIV: EF - Botanics
Impact factor: 3.098, year: 2002

A method for separation of cytokinins from auxin and abscisic acid, which allows further separation ofcylokinin ribotides from cytokinin bases, ribosidcs und glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationie cylokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluled by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of (he method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (R1A) or I1PLC-MS.
Permanent Link: http://hdl.handle.net/11104/0069262