0170645 - UEK-B 20020011 RIV NL eng J - Journal Article
Šafařík, Ivo - Ptáčková, Lucie - Koneracká, M. - Šafaříková, Miroslava - Timko, M. - Kopčanský, P.
Determination of selected xenobiotics with ferrofluid-modified trypsin.
Biotechnology Letters. Roč. 24, - (2002), s. 355-358. ISSN 0141-5492. E-ISSN 1573-6776
R&D Projects: GA MŠMT OC 523.80
Institutional research plan: CEZ:AV0Z6087904
Keywords : ferrofluid-modified trypsin * magnetic fluids * trypsin inhibitors
Subject RIV: EH - Ecology, Behaviour
Impact factor: 0.802, year: 2002

Ferrofluid-modified trypsin has been used for the detection and determination of selected xenobiotics that inhibit trypsin activity. The procedure is useful especially when colored samples or samples containing suspended solid impurities are to be assayed. Ferrofluid-modified trypsin was inhibited by Ag+ and Pb2+, selected dyes (safranin, thionin), bacitracin and 4-aminobenzamidine. Enzymes immobilized on magnetic particles can form a basis of new automated assay procedures for the determination of xenobiotics.
Permanent Link: http://hdl.handle.net/11104/0067729