0162345 - UIACH-O 20010096 RIV GB eng J - Journal Article
Žídková, Jitka - Chmelík, Josef
Determination of saccharides in fruit juices by capillary electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
Journal of Mass Spectrometry. Roč. 36, č. 4 (2001), s. 417-421. ISSN 1076-5174. E-ISSN 1096-9888
R&D Projects: GA MZe EP9410
Institutional research plan: CEZ:AV0Z4031919
Keywords : fruit juices * adulteration * matrix-assisted laser deasorption
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.685, year: 2001

A fast method for the detection of cheap sweeteners is presented. Detecting the adulteration of foods rich in carbohydrates is complicated by the presence of variety of commercial sweeteners that are designed to match exactly the major carbohydrate profiles of these foods. Electrophoretic and mass spectrometric assays for the determination of fruit juice authenticity were developed. Capillary zone electrophoresis with indirect detection was employed to detect adulteration of juice demonstrated by the ratio of the concentrations of major low molecular mass saccharides (glucose, fructose and sucrose). Traces of oligosaccharides, which are not present in the sugar profiles of citrus but are present in inexpensive sweeteners, were evaluated as the other group of target compounds. The fast determination of oligomeric starch hydrolysates in a complex matrix was tested by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and applied to orange juice.
Permanent Link: http://hdl.handle.net/11104/0059663