0162276 - UIACH-O 20010022 RIV US eng J - Journal Article
Radko, S. P. - Šťastná, Miroslava - Chrambach, A.
Polydispersity of liposome preparations as a likely source of peak width in capillary zone electrophoresis.
Journal of Chromatography-B. Roč. 761, č. 1 (2001), s. 69-75. ISSN 0378-4347
Institutional research plan: CEZ:AV0Z4031919
Keywords : polydispersity * liposomes
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 1.911, year: 2001

Negatively charged liposomes consisting of phosphatidylcholine/phosphatidylglycerol/cholesterol in various ratios when subjected to capillary zone electrophoresis (CZE) in Tris-HCl (pH 8) buffer of different concentrations have been shown previously to exhibit a size-dependent migration rate at low ionic strength. The present study, focusing on the peak width under those conditions, shows that the polydispersity of liposomes correlated with, and appears to be a dominant source of, the peak width of the liposomes in CZE in a buffer of low ionic strength (2 to 5 mM Tris-HCl buffer, pH 8) at moderate electric field strengths (200 V cm(-1) or less). This finding, beyond allowing for the analysis of liposome polydispersity by CZE, suggests that the size-dependent fractionation of liposome preparations by a preparative electrophoretic technique such as free-flow electrophoresis is potentially feasible.
Permanent Link: http://hdl.handle.net/11104/0059595