0162271 - UIACH-O 20010016 RIV NL eng J - Journal Article
Jandera, P. - Bunčeková, S. - Mihlbachler, K. - Guiochon, G. - Bačkovská, V. - Planeta, Josef
Fitting adsorption isotherms to the distribution data determined using packed micro-columns for high-performance liquid chromatography.
Journal of Chromatography A. Roč. 925, 1-2 (2001), s. 19-29. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA MŠMT ME 150; GA AV ČR IAA4031802
Grant - others:NATO(XE) OUT-R.LG971480
Institutional research plan: CEZ:AV0Z4031919
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.793, year: 2001

Knowing the adsorption isotherms of the components of a mixture on the chromatographic system used to separate them is necessary for a better understanding of the separation process and for the optimization of the production rate and costs in preparative high-performance liquid chromatography (HPLC). Currently, adsorption isotherms are usually measured by frontal analysis, using conventional analytical columns. Unfortunately, this approach requires relatively large quantities of pure compounds, and hence is expensive, especially in the case of pure enantiomers. In this work, we investigated the possible use of packed micro-bore and capillary HPLC columns for the determination of adsorption isotherms of benzophenone, o-cresol and phenol in reversed-phase systems and of the enantiomers of mandelic acid on a Teicoplanin chiral stationary phase. We found a reasonable agreement between the isotherm coefficients of the model compounds determined on micro-columns and on conventional analytical columns packed with the same material. Both frontal analysis and perturbation techniques could be used for this determination. The consumption of pure compounds needed to determine the isotherms decreases proportionally to the second power of the decrease in the column inner diameter, i.e. 10 times for a micro-bore column (1 mm I.D.) and 100 times for capillary columns (0.32 mm I.D.) with respect to 3.3 mm I.D. conventional columns
Permanent Link: http://hdl.handle.net/11104/0059590