New aspects of buffering with multivalent weak acids in capillary zone electrophoresis: Pros and cons of the phosphate buffer

0162202 - UIACH-O 200033 RIV DE eng J - Journal Article
Gebauer, Petr - Boček, Petr
New aspects of buffering with multivalent weak acids in capillary zone electrophoresis: Pros and cons of the phosphate buffer.
Electrophoresis. Roč. 21, č. 14 (2000), s. 2809-2813. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA AV ČR IAA4031703; GA ČR GA203/99/0044; GA MŠMT VS96021; GA MŠMT VS97014
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.385, year: 2000

This contribution brings a theoretical study of the analytical separation performance of phosphate buffer, serving as model of buffering with multivalent weak acids. Single multivalent weak acids mimic the performance of background electrolytes with multiple co-anionic species for anionic analyses. It is shown that simple phosphate buffers may exhibit up to two regions of symmetry, of fronting as well as of tailing zones on the mobility scale inside the sample window. Moreover they may exhibit a region of enormous electromigration dispersion.
Permanent Link: http://hdl.handle.net/11104/0059521