0157581 - PAU-O 20023056 RIV NL eng J - Journal Article
Maslov, D. A. - Zíková, Alena - Kyselová, Iveta - Lukeš, Julius
A putative novel nuclear-encoded subunit of the cytochrome c oxidase complex in trypanosomatids.
Molecular and Biochemical Parasitology. Roč. 125, 1-2 (2002), s. 113-125. ISSN 0166-6851. E-ISSN 1872-9428
R&D Projects: GA ČR GA204/00/1212
Grant - others:NIH(US) AI40634
Institutional research plan: CEZ:AV0Z6022909
Keywords : cytochrome c oxidase * mitochondrion * kinetoplast
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.911, year: 2002

A relatively large nuclear-encoded polypeptide, designated trCOIV, is found in the cytochrome c oxidase (CO) complex of trypanosomatids. In order to determine if this polypeptide represents a bona fide subunit of the complex, we have characterized the cDNA and the gene for this polypeptide in Leishmania tarentolae. Its nuclear gene has no sequence similarity to mammalian COIV. The trCOIV preprotein has a long mitochondria targeting sequence of 31 residues. The mature polypeptide cofractionates with kinetoplast-mitochondria and its preferential mitochondria localization was confirmed by immunofluorescence and immunoelectron microscopy. Based on the hydropathy plot analysis, the protein lacks pronounced transmembrane domains and likely occupies a peripheral position within the CO complex. The corresponding genes are also present in the sequenced portions of the Trypanosoma cruzi, T. brucei and Leishmania major genomes, and the same polypeptide is found in cytochrome oxidase isolated from procyclic T. brucei and promastigote L. mexicana amazonensis.
Permanent Link: http://hdl.handle.net/11104/0055019