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Different methods of membrane domains isolation result in similar 2-D distribution patterns of membrane domain proteins
- 1.0142571 - FGU-C 20030182 RIV CA eng J - Journal Article
Matoušek, Petr - Hodný, Zdeněk - Švandová, I. - Svoboda, Petr
Different methods of membrane domains isolation result in similar 2-D distribution patterns of membrane domain proteins.
Biochemistry and Cell Biology. Roč. 81, č. 6 (2003), s. 365-372. ISSN 0829-8211. E-ISSN 1208-6002
R&D Projects: GA MŠMT LN00A026
Grant - others:Wellcome Trust(GB) xx
Institutional research plan: CEZ:AV0Z5011922; CEZ:MSM 113100003; CEZ:AV0Z5039906
Keywords : membrane domain * G protein * two-dimensional electrophoresis * GPI-ancored proteins
Subject RIV: CE - Biochemistry
Impact factor: 2.456, year: 2003
The present work compared three different procedures (drastic homogenisation, detergent extraction and alkaline treatment) of membrane domain isolation. Protein composition of membrane domains prepared by different procedures was very similar.
Permanent Link: http://hdl.handle.net/11104/0040272
Number of the records: 1