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Confocal and two-photon microscopy: foundations, applications, and advances

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    0142299 - FGU-C 20020021 RIV US eng M - Monography Chapter
    Kubínová, Lucie - Janáček, Jiří - Krekule, Ivan
    Stereological methods for estimating geometrical parameters of microscopic structure by three-dimensional imaging.
    Confocal and two-photon microscopy: foundations, applications, and advances. Vol. 15. New York: WileyLiss, 2002 - (Diaspro, A.), s. 299-332. ISBN 0-471-40920-0
    R&D Projects: GA AV ČR IAA5011810; GA ČR GA304/01/0257; GA ČR GA304/99/0680; GA MŠk ME 184
    Institutional research plan: CEZ:AV0Z5011922
    Keywords : three-dimensional imagine * confocal microscopy
    Subject RIV: EA - Cell Biology

    Methods for estimating number, length, surface area and volume of microscopical structures, based on evaluation of three-dimensional image data obtained by confocal microscopy, are presented: (i) interactive unbiased stereological methods using virtual spatial grids generated by a computer applied to thick slices of arbitrary orientation; (ii) automatic digital methods based on image processing; (iii) automatic digital methods based on surface triangulation. The application of all methods, using our programs and software modules, is shown on practical examples. The methods are compared from the point of view of their applicability, efficiency and precision.
    Permanent Link: http://hdl.handle.net/11104/0040005

     
     

Number of the records: 1