Dual effects of muscarinic M2 acetylcholine receptors on the synthesis of cyclic AMP in CHO cells: dependence on time, receptor density and receptor agonists

0142132 - FGU-C 20010185 RIV GB eng J - Journal Article
Michal, Pavel - Lysíková, Michaela - Tuček, Stanislav
Dual effects of muscarinic M2 acetylcholine receptors on the synthesis of cyclic AMP in CHO cells: dependence on time, receptor density and receptor agonists.
British Journal of Pharmacology. Roč. 132, č. 6 (2001), s. 1217-1228. ISSN 0007-1188. E-ISSN 1476-5381
R&D Projects: GA ČR GA309/99/0214; GA AV ČR IAA7011910
Institutional research plan: CEZ:AV0Z5011922
Keywords : cyclic AMP * muscarinic receptors * CHO cells
Subject RIV: ED - Physiology
Impact factor: 3.502, year: 2001

Muscarinic M2 receptors normally inhibit the production of cyclic AMP via Gi proteins, but a stimulatory component occurs in their effect at high agonist concentrations, believed to be based on the activation of Gs proteins. We investigated the conditions which determine the occurrence and extent of the stimulatory component in CHO cells stably expressing muscarinic M2 receptors. Biphasic concentration-response curves (decline followed by return towards control values) were obtained after 10 min incubation with carbachol, oxotremorine-M, acetylcholine, arecoline and arecaidine propargyl ester, but the upward phase was missing with oxotremorine, methylfurmethide, furmethide and pentylthio-TZTP. Shortening the incubation favoured the occurrence of the stimulatory component. Carbachol (1 mM) and oxotremorine-M (1 mM) brought about net stimulation (above 100
Permanent Link: http://hdl.handle.net/11104/0039841