Number of the records: 1
Heat-inducible transgenic expression in the silkmoth Bombyx mori
- 1.0135886 - ENTU-I 20023106 RIV US eng J - Journal Article
Uhlířová, Miroslava - Asahina, Masako - Riddiford, L. M. - Jindra, Marek
Heat-inducible transgenic expression in the silkmoth Bombyx mori.
Development Genes and Evolution. Roč. 212, - (2002), s. 145-151. ISSN 0949-944X. E-ISSN 1432-041X
R&D Projects: GA AV ČR IAB5007002
Institutional research plan: CEZ:AV0Z5007907
Keywords : Germline transformation * piggyBac transposon
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.339, year: 2002
We report successful germline transformation of the silkmoth Bombyx mori with the piggyBas transposon. Besides the Pax-6driven GFP selectable marker, we inserted into the Bombyx genome a heat-inducible hsp 70 cassette from Drosophila. For the first time we show that a transgene can be induced in vivo by heat shock in a non-drosophilid insect, establishing a system for future transgenic studies.
Permanent Link: http://hdl.handle.net/11104/0033662
Number of the records: 1