Arrangement of chromosome 11 and 22 territories, EWSR1 and FLI1 genes, and other genetic elements of these chromosomes in human lymphocytes and Ewing sarcoma cells

0127067 - BFU-R 20033155 RIV US eng J - Journal Article
Taslerová, R. - Kozubek, Stanislav - Lukášová, Emilie - Jirsová, Pavla - Bártová, Eva - Kozubek, Michal
Arrangement of chromosome 11 and 22 territories, EWSR1 and FLI1 genes, and other genetic elements of these chromosomes in human lymphocytes and Ewing sarcoma cells.
Human Genetics. Roč. 112, č. 2 (2003), s. 143-155. ISSN 0340-6717. E-ISSN 1432-1203
R&D Projects: GA MZd NC5955; GA AV ČR IBS5004010; GA ČR GA301/01/0186
Institutional research plan: CEZ:AV0Z5004920
Keywords : high-resolution cytometry * human leukemic cells * Ewing sarcoma cells
Subject RIV: BO - Biophysics
Impact factor: 4.022, year: 2003

Standard and repeated fluorescence in situ hybridization and high-resolution cytometry were used to study topographical parameters of chromosome 11 and 22 territories, EWSR1 and FLI1 genes, and other genetic elements of these chromosomes in human lymphocytes and Ewing sarcoma cells. HSA 11 and its elements (BCL1, FLI1, centromere) were found, on average, more peripherally in comparison with HSA 22 and investigated elements (BCR, EWSR1, centromere). After the elimination of fluctuations of chromosome territories in nuclear volume, it was found that genetic elements in most cases adhered to their territories. The investigated genetic elements of HSA 11 were found close to each other relative to the large molecular lengths among them. This finding indicates a higher degree of chromatin condensation of at least a part of HSA 11 compared with HSA 22.
Permanent Link: http://hdl.handle.net/11104/0025290