Circular dichroism spectroscopy of conformers of (guanine + adenine) repeat strands of DNA

0127058 - BFU-R 20033146 RIV US eng J - Journal Article
Kejnovská, Iva - Kypr, Jaroslav - Vorlíčková, Michaela
Circular dichroism spectroscopy of conformers of (guanine + adenine) repeat strands of DNA.
Chirality. Roč. 15, č. 7 (2003), s. 584-592. ISSN 0899-0042. E-ISSN 1520-636X
R&D Projects: GA AV ČR IAA4004201; GA ČR GA204/01/0561
Institutional research plan: CEZ:AV0Z5004920
Keywords : DNA conformation * (guanine + adenine) repeats * homoduplexes
Subject RIV: BO - Biophysics
Impact factor: 1.793, year: 2003

(Guanine+adenine) strands of DNA are known to associate into guanine tetraplexes, homodimerize into parallel or antiparallel duplexes, and fold into a cooperatively melting single strand resembling the protein alpha helix. Using CD spectroscopy and other methods, we studied how this conformational polymorphism depended on the primary structure of DNA. The study showed that d(GGGA)(5) and d(GGA)(7) associated into homoduplexes at low salt or in the presence of LiCl but were prone to guanine tetraplex formation, especially in the presence of KCl.
Permanent Link: http://hdl.handle.net/11104/0025281