Multiple biological effects of inhibitors of arachidonic acid metabolism on human keratinocytes

0126820 - BFU-R 20023120 RIV DE eng J - Journal Article
Pacherník, Jiří - Hampl, Aleš - Souček, Karel - Kovaříková, Martina - Andrysík, Zdeněk - Hofmanová, Jiřina - Kozubík, Alois
Multiple biological effects of inhibitors of arachidonic acid metabolism on human keratinocytes.
Archives of Dermatological Research. Roč. 293, č. 12 (2002), s. 626-633. ISSN 0340-3696. E-ISSN 1432-069X
R&D Projects: GA ČR GA524/99/0694; GA AV ČR IBS5004009; GA ČR GA524/98/0190
Institutional research plan: CEZ:AV0Z5004920
Keywords : lipoxygenase * keratinocytes * cell cycle
Subject RIV: BO - Biophysics
Impact factor: 1.452, year: 2002

The effects of several structurally different inhibitors of AA metabolism in the human keratinocyte HaCaT cell line were examined. While eicosatetraynoic acid (ETYA), nordihydroguaiaretic acid (NDGA), esculetin and MK-886 reduced proliferation and induced apoptosis of HaCaT cells, the cyclooxygenase inhibitors indomethacin and piroxicam had no such effects. Esculetin induced redistribution of F-actin accompanied by increased cell adhesion and size. Our findings indicate that, in addition to their ability to inhibit cell proliferation and to induce apoptosis, lipoxygenase inhibitors and/or ETYA may also elicit other important physiological responses in HaCaT keratinocytes.
Permanent Link: http://hdl.handle.net/11104/0025048