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Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reactions applications

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    0105806 - UMCH-V 20043377 RIV NL eng J - Journal Article
    Španová, A. - Horák, Daniel - Soudková, E. - Rittich, B.
    Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reactions applications.
    [Magnetické hydrofilní polymerní mikročástice na bázi methakrylátu pro použití v PCR.]
    Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 800, 1-2 (2004), s. 27-32. ISSN 1570-0232. E-ISSN 1873-376X.
    [International Symposium on Separations in the Bioscience /3./. Moscow, 13.05.2003-18.05.2003]
    R&D Projects: GA ČR GA202/02/1361; GA ČR GA525/02/0287
    Institutional research plan: CEZ:AV0Z4050913
    Keywords : polymerase chain reaction inhibition * magnetic microspheres * methacrylates
    Subject RIV: BM - Solid Matter Physics ; Magnetism
    Impact factor: 2.176, year: 2004

    Magnetic hydrophilic non-porous P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA microspheres were prepared by dispersion (co)polymerization of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of several kinds of magnetite. It was found that some components used in the preparation of magnetic carriers interfered with polymerase chain reaction (PCR).

    Neporézní magnetické hydrofilní P(HEMA-co-EDMA), P(HEMA-co-GMA) a PGMA mikročástice byly připraveny disperzní (ko)polymerizací 2-hydroxyethyl-methakrylátu (HEMA) a ethylen-dimethakrylátu (EDMA) nebo glycidyl-methakrylátu (GMA) v přítomnosti několika druhů magnetitu.
    Permanent Link: http://hdl.handle.net/11104/0012999


     
     
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