Bio-compatibility of ion beam-modified and RGD-grafted polyethylene

0105594 - UJF-V 20043143 RIV NL eng J - Journal Article
Ročková-Hlaváčková, K. - Švorčík, V. - Bačáková, L. - Dvořánková, B. - Heitz, J. - Hnatowicz, Vladimír
Bio-compatibility of ion beam-modified and RGD-grafted polyethylene.
[Biokompatibilita polyethylenu modifikovaného ozářením ionty a roubováním RGD.]
Nuclear Instruments & Methods in Physics Research Section B. Roč. 225, č. 3 (2004), s. 275-282. ISSN 0168-583X. E-ISSN 1872-9584
R&D Projects: GA AV ČR IAA5011301
Institutional research plan: CEZ:AV0Z1048901
Keywords : modified polyethylene * ion beam * RGD grafting
Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders
Impact factor: 0.997, year: 2004

Polyethylene (PE) was implanted with 15 keV Ar+ and Kr+ ions to the fluences from 3 x 10(12)-1 x 10(15) cm(-2) and subsequently grafted with amino acid sequence Arg-Gly-Asp (RGD), i.e. a minimum adhesion motif recognized by integrin receptors on cells. The structural changes of PE were studied using goniometric technique, UV-VIS, LIF spectroscopy and by measuring specimen electrical resistance. The adhesion and proliferation of mouse embryonic fibroblasts 3T3 on the modified PE were studied under in vitro conditions. Addition of RGD sequence onto double bonds created by the ion irradiation on polymeric chain was observed. The adhesion and proliferation of the 3T3 cells is increased by ion implantation and additionally also by RGD grafting

Polyethylen (PE) byl implantován ionty Ar a Kr s energií 15 keV a následně roubován sekvencí aminokyselin Arg-Gly-Asp (RGD). Strukturní změny a vlastnosti modifikovaného PE byly sledovány různými metodami. Adhese a proliferacce myších embryonálních fibroblastů 3T3 na modifikovaném PE byly sledovány in vitro. Adhese a proliferace buněk se zvyšují iontovým ozářením a roubováním
Permanent Link: http://hdl.handle.net/11104/0012830