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Cadmium tolerance and accumulation in transgenic tobacco plants with a yeast metallothionein combined with a polyhistidine tail

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    0100794 - UOCHB-X 20043019 RIV GB eng J - Journal Article
    Pavlíková, D. - Macek, Tomáš - Macková, M. - Száková, J. - Balík, J.
    Cadmium tolerance and accumulation in transgenic tobacco plants with a yeast metallothionein combined with a polyhistidine tail.
    [Schopnost tolerovat a akumulovat kadmium u transgenních rostlin tabáku nesoucích kvasničný metalothionein ve fúzi s polyhistidinovou kotvou.]
    International Biodeterioration & Biodegradation. Roč. 54, - (2004), s. 233-237. ISSN 0964-8305. E-ISSN 1879-0208
    R&D Projects: GA ČR GA526/02/0293
    Institutional research plan: CEZ:AV0Z4055905
    Keywords : transgenic tobacco * cadmium * accumulation
    Subject RIV: EI - Biotechnology ; Bionics
    Impact factor: 0.835, year: 2004

    Cadmium accumulation and tolerance were tested using tobacco, bearing the transgene coding for polyhistidine cluster, combined with yeast metallothionein (HisCUP). Addition of 5.4 mg cadmium per litre induced symptoms of cadmium toxicity in the control tobacco, and the highest dose of cadmium was lethal. HisCUP showed increasing resistance to the stress response induced by cadmium and doubled Cd accumulation in harvestable parts

    Akumulace a tolerance vůči kadmiu byla sledována u tabáku s vneseným transgenem kodujícím polyhistidinovou kotvu ve fúzi s kvasničným metalothioneinem (HisCUP). Přídavek 5,4 mg kadmia na litr indukoval symptomy toxicity v kontrolních rostlinách tabáku, nejvyšší dávka kadmia byla letální. HisCUP vykazuje zvýšenou rezistenci vůči stresu indukovanému kadmiem a dvojnásobnou akumulaci Cd v nadzemních částech
    Permanent Link: http://hdl.handle.net/11104/0008283

     
     

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