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Rpg1p/eIF3a associates with mitrochondria in saccharomyces cerevisiae

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    0100376 - MBU-M 20040066 RIV CZ eng C - Conference Paper (international conference)
    Hašek, Jiří - Janatová, Ivana - Špryngar, Martin - Wolinski, H. - Kohlwein, S. D.
    Rpg1p/eIF3a associates with mitrochondria in saccharomyces cerevisiae.
    [Rpg1p/eIF3a se spojuje s mitochondriemi v Saccharomyces cerevisiae.]
    Cytoskeletal Club /12./. Vranovská Ves, 2004, s. 14.
    [Cytoskeltal Club /12./. Vranovská Ves (CZ), 28.04.2004-30.04.2004]
    R&D Projects: GA ČR GA204/02/1424
    Grant - others:GA-(CZ) 2004/29
    Institutional research plan: CEZ:AV0Z5020903
    Keywords : rpg1 * sla2p
    Subject RIV: EE - Microbiology, Virology

    RPG1/TIF32, the essential gene of Saccharomyces cerevisiae, encodes eIF3a subunit of the translation initiation factor 3. We show here that both temperature sensitive mutant of Rpg1 strongly differ in their distribution at permissive temperature, distribution of the Rpg1-1-GFP fusion is cytosolic, the fusion protein Rpg1-2-GFP was accumulated in large cytosolic aggregates. Using the life image analysis with confocal laser scanning microscope we found that these granules could be inherited to daughter cells and obviously associate with mitochondria.

    RPG1/TIF32 je esenciálním genem kvasinky S.cerevisiae. Ukázali jsme, že ts mutace rpg1-1 a rpg1-2 ovlivnují distribuci tohoto proteinu. Zvláště intragenově značená verse mutantu rpg1-2 se akumuluje v granulích i v permisivní teplotě a tyto granule asociují s mitochondriemi a jsou děděny.
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