Intestinal microflora does not affect tolerance induction to birch pollen allergen in mice

0076165 - MBÚ 2007 RIV SIGLE SE eng C - Conference Paper (international conference)
Kozáková, Hana - Repa, A. - Štěpánková, Renata - Hrnčíř, Tomáš - Hudcovic, Tomáš - Tlaskalová, Helena - Wiedermann, U.
Intestinal microflora does not affect tolerance induction to birch pollen allergen in mice.
[Střevní mikroflóra neovlivňuje navození tolerance k pylu břízy.]
Microbial Ecology in Health and Disease. Švédsko: Taylor and Francis Group, 2006, s. 232-232.
[Gnotobiology Symposium 2005. Tokio (JP), 20.06.2005-24.06.2005]
R&D Projects: GA ČR GA303/05/2249
Institutional research plan: CEZ:AV0Z50200510
Keywords : germ-free * tolerance
Subject RIV: EE - Microbiology, Virology

The aim of our work was to study the development of mucosal tolerance to major allergic component of birch pollen - Bet v1 in relationship with presence of intestinal microflora. Germ-free (GF) or conventionally reared (CV) BALB/c mice were intragastrically or intranasally pre-treated with Bet v 1 prior to sensitization performed by subcutaneous injections of Bet v 1 with aluminum hydroxide as adjuvans. Oral as well as intranasal tolerance induction led to a significant reduction of allergen-specific antibody levels and IgE mediated basophil degranulation, as well as cytokine production in vitro in both GF and CV mice

Cílem práce bylo studovat vliv střevní mikroflóry na navození slizniční tolerance k pylu břízy. Bezmikrobní a konvenční myši kmene BALB/c byly sondovány Bet v 1 intragastricky a intranasálně a po té byly senzitizovány subkutálně Bet v 1 s hydroxidem sodným. Byla navozena tolerance nezávisle na přítomnosti bakterií, která se odrazila ve snížené hladině IgE a změněné cytokinové produkci
Permanent Link: http://hdl.handle.net/11104/0143336