Methods for compensation of the light attenuation with depth of images captured by a confocal microscope

0049753 - FGÚ 2007 RIV US eng J - Journal Article
Čapek, Martin - Janáček, Jiří - Kubínová, Lucie
Methods for compensation of the light attenuation with depth of images captured by a confocal microscope.
[Metody kompenzace útlumu jasu s hloubkou obrazů získaných konfokálním mikroskopem.]
Microscopy Research and Technique. Roč. 69, č. 8 (2006), s. 624-635. ISSN 1059-910X. E-ISSN 1097-0029
R&D Projects: GA MŠMT(CZ) LC06063; GA AV ČR(CZ) IAA100110502; GA AV ČR(CZ) IAA600110507; GA AV ČR(CZ) IAA500200510; GA AV ČR(CZ) KJB6011309; GA ČR(CZ) GA304/05/0153
Institutional research plan: CEZ:AV0Z50110509
Keywords : image enhancement * confocal microscopy * histogram warping
Subject RIV: JC - Computer Hardware ; Software
Impact factor: 1.680, year: 2006

A confocal laser scanning microscope (CLSM) enables us to capture images from a biological specimen in different depths and obtain a series of precisely registered fluorescent images. But images captured from deep layers of the specimen may be darker than images from the topmost layers due to light loss distortions. This effect causes difficulties in subsequent analysis of biological objects. We propose a solution using two approaches: either an online method working already during image acquisition, or an offline method assisting as a post-processing step. In the online method the gain value of a photomultiplier tube of a CLSM is controlled according to the difference of mean image intensities between the reference and currently acquired image. The offline method consists of two stages. In the first stage a standard histogram improving brightness and contrast is created from all images in the series. In the second stage image histograms are warped according to this standard histogram

Konfokální laserový mikroskop umožňuje získat 3D obrazy biologického vzorku. Snímání je však zatíženo útlumem jasu a kontrastu obrazů s hloubkou zaostření. Článek popisuje metody kompenzace tohoto útlumu jednak během samotného snímaní a jednak úpravou obrazů po nasnímání
Permanent Link: http://hdl.handle.net/11104/0140053