HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets

0043244 - MBÚ 2007 RIV SIGLE NL eng J - Journal Article
Sheshko, Valeria - Hejnová, Jana - Řeháková, Z. - Šinkora, J. - Faldyna, M. - Alexa, P. - Felsberg, Jürgen - Němcová, R. - Bomba, A. - Šebo, Peter
HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets.
[Vyřazení HlyA vede k bezpečnější variantě kmene Escherichia coli A0 34/86 s nezměněnými kolonizačními schopnostmi.]
FEMS Immunology and Medical Microbiology. Roč. 48, - (2006), s. 257-266. ISSN 0928-8244
R&D Projects: GA ČR GA523/03/0186
EU Projects: European Commission(XE) 503240 - MUVAPRED
Institutional research plan: CEZ:AV0Z50200510
Keywords : escherichia coli * haemolysin * colonization
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.281, year: 2006

Escherichia coli A0 34/86 (O83:K24:H31) has been successfully used for prophylactic and therapeutic intestinal colonization of premature and newborn infants, with the aim of preventing nosocomial infections. Although E. coli A0 34/86 was described as a nonpathogenic commensal, partial sequencing revealed that its genome harbours gene clusters highly homologous to virulence determinants of different types of E. coli, including closely linked genes of the a-haemolysin operon (hlyCABD) and for the cytotoxic necrotizing factor (cnf1). A haemolysin-deficient mutant (hlyA) of E. coli A0 34/86 was generated and its colonization capacity was determined. The results show that a single dose of the A0 34/86 wild-type or hlyA strains resulted in efficient intestinal colonization of newborn conventional piglets, and that this was still considerable after several weeks

Kmen Escherichia coli A0 34/86 (O83:K24:H31) je úspěšně používán pro preventivní kolonizaci novorozenců. V této práci ukazujeme, že jeho bezpečnost lze ještě vylepšit odstraněním genu pro hemolyzín, což neovlivní kolonizační schopnosti kmene v prasečím experimentálním modelu
Permanent Link: http://hdl.handle.net/11104/0136286