DNA methylation pattern in pig in vivo produced embryos

0042040 - ÚŽFG 2007 RIV DE eng J - Journal Article
Fulka, Josef - Fulková, H. - Slavík, Tomáš - Okada, K. - Fulka Jr., J.
DNA methylation pattern in pig in vivo produced embryos.
[Prubeh metylace DNA u prasecich embryi produkovanych in vivo.]
Histochemistry and Cell Biology. Roč. 126, č. 2 (2006), s. 213-217. ISSN 0948-6143. E-ISSN 1432-119X
R&D Projects: GA ČR GESTE/05/E004
Institutional research plan: CEZ:AV0Z50450515
Keywords : methylation * DNA * embryos
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.220, year: 2006

DNA methylation/demethylation pattern, determined by 5-methylcytosine (5-MeC) immunostaining, was evaluated in porcine “in vivo” produced embryos from zygote up to the blastocyst stage. In one-cell stage embryos, only the maternal pronucleus showed a positive labeling whilst the paternal pronucleus showed almost no labeling. The intensity of labeling is high until the late morula stage. Blastocysts containing less than 100 cells showed the same intensity of labeling in both the inner cell mass (ICM) nuclei and the trophectodermal (TE) cell nuclei. Interestingly, with further cell multiplication, cells of the ICM became more intensively labeled when compared to TE cells. This distinct methylation pattern is even more profound in blastocysts containing about 200–300 cells and is not caused by the difference in the cell volume of ICM and TE cells.

Uroven metylace DNA byla studovana pomoci protilatky proti-5-MeC behem vyvoje prasecich embryi od stadia zygoty do stadia expandovane blasocysty.Vysledky ukazaly,ze u zygoty vykazuje positivni znaceni pouze samici prvojadro, zatim co samci pronucleus zustava temer neznacen. Po syngamii a naslednem deleni pretrvava vysoka uroven znaceni az do stadia moruly. U blastocysty s obsahem mene nez 100 bunek ma znaceni stejnou intensitu u bunek embryoblastu a trofoblastu. Pozdeji dochazi k navyseni urovne znaceni u bunek embryoblastu, bunky trofoblastu zustavaji znacene mene intensivne.Rozdil v urovni znaceni ve prospech bunek embryoblastu se dale vyrazne zvysuje u blastocyst s obsahem 200-300 bunek a nezavisi na objemu hodnocenych bunek.
Permanent Link: http://hdl.handle.net/11104/0135358