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Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants

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    0028014 - UMBR-M 2006 RIV NL eng J - Journal Article
    Vrba, Lukáš - Matoušek, Jaroslav
    Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants.
    [Exprese modifikovaného genu pro 7SL RNA v transgenních rostlinách druhu Solanum tuberosum.]
    Biologia Plantarum. Roč. 49, - (2005), 371-380. ISSN 0006-3134. E-ISSN 1573-8264
    Institutional research plan: CEZ:AV0Z50510513
    Keywords : transgenic plants * Solanum tuberosum
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 0.792, year: 2005

    A modified plant 7SL RNA gene from Arabidopsis thaliana designated AHIIA63M was introduced into potato plants via Agrobacterium-mediated transformation. Special binary vectors with matrix attachment region (MAR) elements had to be used for transformation to insulate polII and polIII promoters within T-DNA.

    Agrobaktériem zprostředkovaná transformace byla použita pro vnesení modifikovaného genu pro 7SL RNA z Arabidopsis do rostlin bramboru. K ochraně promotorů polII a polIII v T-DNA musely být k transformaci použity speciální binární vektory obsahující tzv. matric attachment region (MAR) oblast.
    Permanent Link: http://hdl.handle.net/11104/0118003

     
     
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