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Molecular characterization of phytoplasmas in lilies with fasciation in the Czech Republic
- 1.0026847 - UMBR-M 2006 RIV GB eng J - Journal Article
Bertaccini, A. - Fránová, Jana - Botti, S. - Tabanelli, D.
Molecular characterization of phytoplasmas in lilies with fasciation in the Czech Republic.
[Molekulární charakterizace fytoplazem u fasciovaných lilií v České republice.]
FEMS Microbiology Letters. Roč. 249, - (2005), s. 79-85. ISSN 0378-1097. E-ISSN 1574-6968
R&D Projects: GA AV ČR(CZ) IBS5051014
Institutional research plan: CEZ:AV0Z50510513
Keywords : phytoplasma * Lilium spp. * molecular characterization
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.057, year: 2005
Lilium spp. with symptoms of severe observed in Southern and central Bohemian during the period 1999-2003. Nucleid acids extracted from symptomatic and asymptomatic plants were used nested-PCR assay with primers amplifying 16S-23SrRNA sequences specific for phytoplasmas. The sequences obtained were closely related, respectively, to operon A and B ribosomal sequences of the clover phyllody phytoplasma.
V rostlinách lilií se silnými příznaky fasciace byly detekovány fytoplazmy podskupin 16SrI-B a 16SrI-C. Rostliny infikované fytoplazmou podskupiny 16SrI-C vykázaly po amplifikaci s primery R16F2/R2 a následném štěpení enzymem TruI odlišné profily. Sekvenční analýza potvrdila přítomnost operonu A a operonu B fytoplazmy fylodie jetele (16SrI-C). V bezpříznakových rostlinách lilií cv. Sunray byla prokázána přítomnost fytoplazem podskupiny 16SrI-B.
Permanent Link: http://hdl.handle.net/11104/0117016
Number of the records: 1