In vitro inhibitory effects of thymol and quinones of Nigella sativa seeds on cyclooxygenase-1-and -2-catalyzed prostaglandin E2 biosyntheses

0022309 - ÚOCHB 2006 RIV DE eng J - Journal Article
Maršík, Petr - Kokoška, L. - Landa, P. - Nepovím, Aleš - Soudek, Petr - Vaněk, Tomáš
In vitro inhibitory effects of thymol and quinones of Nigella sativa seeds on cyclooxygenase-1-and -2-catalyzed prostaglandin E2 biosyntheses.
[Inhibiční efekt tymolu a chinonů ze semen Nigella sativa na biosyntézu prostaglandinu E2 katalyzovanou cyklooxgenázami -1 a -2 in vitro.]
Planta medica. Roč. 71, - (2005), s. 739-742. ISSN 0032-0943. E-ISSN 1439-0221
R&D Projects: GA ČR(CZ) GA525/02/0257; GA MŠMT(CZ) 1P04OC926.001
Institutional research plan: CEZ:AV0Z40550506
Keywords : Nigella sativa * thymol * thymoquinone
Subject RIV: CE - Biochemistry
Impact factor: 1.628, year: 2005

Dithymoquinone, thymohydroquinone, thymol and thymoquinone, the compounds derived from N. sativa seeds, were investigated for their in vitro anti-inflammatory activities using cyclooxygenase-1 (COX-1) and-2 (COX-2) assays. Thymol was the most active against COX-1 with IC50 value of 0.2 .mu.M, while thymohydroquinone and thymoquinone exhibited the strongest inhibitory effect on COX-2 with IC50 values of 0.1 and 0.3 .mu.M, respectively.

Protizánětlivý účinek dithymochinonu, thymohydrochinonu, thymolu a thymochinonu obsažených v semenech N. sativa byl testován prostřednictvím inhibice aktivity cyklooxygenázy-1 (COX-1) a -2 (COX-2) in vitro. Nejvyšší inhibiční aktivitu vůči COX-1 vykazoval thymol (IC50 = 0,2 .mu.M), zatímco COX-2 byla nejvýrazněji inhibována thymohydrochinonem (IC50 = 0,1 .mu.M) a thymochinonem (IC50 = 0,3 .mu.M).
Permanent Link: http://hdl.handle.net/11104/0111060