Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates

0022006 - MBÚ 2006 RIV SIGLE DE eng J - Journal Article
Nováková, Linda - Přenosilová, Lenka - Pallová, Petra - Janeček, Jiří - Novotná, Jana - Ulrych, Aleš - Echenique, J. - Trombe, M.-C. - Branny, Pavel
Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates.
[Charakterizace serin/threonin proteinkinázy a fosfatázy eukaryotního typu u Streptococcus pneumoniae a určení kinázových substrátů.]
FEBS Journal. Roč. 272, - (2005), s. 1243-1254. ISSN 1742-464X. E-ISSN 1742-4658
R&D Projects: GA ČR GA204/99/1534; GA ČR GA204/02/1423
Grant - others:UK 188/2004/B-BIO/PrF
Institutional research plan: CEZ:AV0Z50200510
Keywords : phosphoglucosamine mutase * phosphoproteome * protein phosphatase
Subject RIV: EE - Microbiology, Virology

Detailed biochemical studies were performed with eukaryotic-type serine/threonine protein kinase StkP and phosphoprotein phosphatase PhpP of Streptococcus pneumoniae. Analysis of phosphoproteome maps of both wild type and stkP null mutant strains revealed two possible substrates for StkP. The evidence is presented that StkP can phosphorylate in vitro phosphoglucosamine mutase GlmM which catalyzes the first step in the biosynthetic pathway leading to the formation of UDP-N-acetylglucosamine, an essential common precursor to cell envelope components

Byla provedena detailní biochemická studie serin/threoninové proteinkinázy StkP a proteinfosfatázy PhpP eukaryotního typu u Streptococcus pneumoniae. Analýzou fosfoproteomových map výchozího kmene a mutanta byly identifikovány dva potenciální substráty. Jeden z nich.fosfoglukosaminmutasa katalyzuje tvorbu UDP-N-acetylglukosaminu, prekurzoru složek buněčné stěny
Permanent Link: http://hdl.handle.net/11104/0110827