0619755 - ÚIACH 2026 IT eng A - Abstract
Moravcová, Dana - Čmelík, Richard - Planeta, Josef - Gogaľová, Zuzana - Šesták, Jozef
Proteomic analysis on short monolythic columns.
41st Informal Meeting on Mass Spectrometry. 2025. s. 114-114.
[Informal Meeting on Mass Spectrometry /41./. 04.05.2025-07.05.2025, Trentino]
R&D Projects: GA MV(CZ) VB02000015
Institutional support: RVO:68081715
Keywords : short monolithic columns * tryptic peptides * mass spectrometry
OECD category: Analytical chemistry
Result website:
https://www.imms25.it/photo/useruploads/media/BOOK_OF_ABSTRACTS_%2041st_IMMS.pdf

We present an application of short monolithic columns based on polyhedral oligomeric silsesquioxane methacrylate and stearyl methacrylate or zwitterionic sulfobetaine monomers for the gradient separation of peptides. The HPLC analyses of peptides originated from tryptic digests of bovine serum albumin and ovalbumin were performed on a portable chromatographic system assembled in our laboratory and connected to ESI-MS instrument. The monolithic capillary columns (0.32 × 50 mm) were prepared in fused silica capillaries when the polymerization mixture was optimized to achieve high selectivity, efficiency, and favorable permeability. Then, the prepared columns were applied for the fast and effective gradient separation of peptides and peptide mixtures under RPLC or HILIC conditions. The number of detected peptides was comparable on both types of monolithic columns.
Permanent Link: https://hdl.handle.net/11104/0366399